Advertisements
Advertisements
प्रश्न
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Advertisements
उत्तर
On finding the palindrome, the endonuclease binds to the DNA.
- It cuts the opposite strands of DNA, but between the same bases on both the strands & forms STICKY ENDS. This sticky ends facilitate the action of enzyme DNA ligase and help in the formation of recombination DNA.
APPEARS IN
संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Which of the following radioisotope is not suitable for DNA labeling based studies?
'Restriction' in restriction enzyme refers to
'Restriction' in Restriction enzyme refers to ______.
Which of the following bacteria is not a source of restriction endonuclease?
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
