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प्रश्न
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
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उत्तर
On finding the palindrome, the endonuclease binds to the DNA.
- It cuts the opposite strands of DNA, but between the same bases on both the strands & forms STICKY ENDS. This sticky ends facilitate the action of enzyme DNA ligase and help in the formation of recombination DNA.
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संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Which of the following radioisotope is not suitable for DNA labeling based studies?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
Which of the following bacteria is not a source of restriction endonuclease?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the importance of elution in this process.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
