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प्रश्न
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
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उत्तर
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
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संबंधित प्रश्न
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
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Give a schematic representation of the transformation of a pro-insulin into insulin.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
How many amino acids are arranged in the two chains of Insulin?
The site of production of ADA the body is ______.
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as ______
A patient is suffering from ADA deficiency. Can he be cured? How?
