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प्रश्न
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
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उत्तर
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संबंधित प्रश्न
Write the function of adenosine deaminase enzyme.
State the role of C-peptide in human insulin.
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
Expand the Given and mention one application of each:
ELISA
Explain how insulin can be produced using recombinant DNA technology.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Which one of the following statements is true regarding DNA polymerase used in PCR?
Explain how “Rosie” is different from a normal cow.
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
The site of production of ADA the body is ______.
Choose the correct option regarding retrovirus.
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
What is a recombinant DNA vaccine? Give two examples.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Discuss briefly how a probe is used in molecular diagnostics.
How is a mature, functional insulin hormone different from its prohormone form?
List the advantages of recombinant insulin.
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.
Name the technique used to detect the presence of HIV in the body of an individual, Justify the principle associated with this technique.
Illustrate using any one example of transgenic bacteria.
Atherosclerosis can be treated with recombinant protein called ______
Choose the INCORRECT statement.
Which type of vaccine uses an oral formulation produced using Bacillus subtilis?
The first edible vaccine was developed in tobacco plants to express which specific antigen?
Why is the Polymerase Chain Reaction (PCR) preferred over conventional culture and serological methods for early disease diagnosis?
