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प्रश्न
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
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उत्तर
Conventionally, Insulin was isolated and refined from the pancreas of pigs and cows to treat diabetic patients. Though it is effective, due to minor structural changes, the animal insulin caused allergic reaction in a few patients.
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संबंधित प्रश्न
Write the function of adenosine deaminase enzyme.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
The first clinical gene therapy was done for the treatment of ________.
How many amino acids are arranged in the two chains of Insulin?
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
What are DNA vaccines?
The hormone insulin is secreted by:
Which of the following is a neuropeptide hormone?
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
ELISA is based on:
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
What is a recombinant DNA vaccine? Give two examples.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
Define Antigen and Antibody. Name any two diagnostic kits based upon them.
A patient is suffering from ADA deficiency. Can he be cured? How?
List the advantages of recombinant insulin.
A cell-free method of amplifying DNA first developed in the mid 1980's revolutionised the field of biotechnology, Name the method and explain the basic steps of the technique involved.
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
