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प्रश्न
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
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उत्तर
Genetically Engineered Insulin:
- Insulin contains two short polypeptide chains: chain A and chain B linked together by disulphide bridges.
- In mammals, insulin is synthesized as a pro-hormone. It contains an extra stretch called C-peptide.
- C-peptide is absent in the mature insulin and is removed during maturation into insulin.
- Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly in 1983. It prepared two DNA sequences corresponding to A and B, chains of human insulin, and introduced them in plasmids of E. coli for production.
The A and B chains produced were separated, extracted, and combined, by creating disulfide bonds to form human insulin.
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संबंधित प्रश्न
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
Which of the following is a neuropeptide hormone?
The site of production of ADA the body is ______.
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Now a days it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize its complimentary DNA in a clone of cells, followed by its detection using autoradiography because ______
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
A host cell must be made competent, before it is able to receive an rDNA. Justify.
Illustrate using any one example of transgenic bacteria.
