Advertisements
Advertisements
प्रश्न
Describe the role of high temperature.
Advertisements
उत्तर
The first step of PCR is denaturation. In this step, high temperature is required for denaturation of the DNA strands i.e. separating the double-stranded DNA into single strands. Since the two strands are held together by hydrogen bonding, a high temperature is required to break down these bonds and separate the two strands.
APPEARS IN
संबंधित प्रश्न
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
What is a recombinant DNA vaccine? Give two examples.
How is a mature, functional insulin hormone different from its prohormone form?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Why is the Polymerase Chain Reaction (PCR) preferred over conventional culture and serological methods for early disease diagnosis?
