Advertisements
Advertisements
Question
Describe the role of high temperature.
Advertisements
Solution
The first step of PCR is denaturation. In this step, high temperature is required for denaturation of the DNA strands i.e. separating the double-stranded DNA into single strands. Since the two strands are held together by hydrogen bonding, a high temperature is required to break down these bonds and separate the two strands.
APPEARS IN
RELATED QUESTIONS
Consult the internet and find out how to make orally active protein pharmaceuticals. What is the major problem to be encountered?
What is gene therapy? Name the first clinical case in which it was used.
Which one of the following statements is true regarding DNA polymerase used in PCR?
Vaccines that use components of a pathogenic organism rather than the whole organism are called ____________.
If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
C-peptide of human insulin is ______.
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
