Advertisements
Advertisements
Question
Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and the chemistry of oil?
Advertisements
Solution
rDNA technology is a genetic engineering approach that combines DNA from two separate sources to create recombined or recombinant DNA (rDNA). Oils consist of glycerol and fatty acids. To make oil-free seeds, genes coding for glycerol or fatty acids should be discovered, and nucleotide sequences complementary to their sequences should be introduced close to these genes in the endosperm’s early cells. During transcription, these complementary sequences produce antisense RNAs similar to those made by the glycerol or fatty acid genes, which effectively turn off these genes. This will result in the creation of oil-free seeds. Since glycerol is a common component of all oils and diverse fatty acids interact with glycerol to form oils, it will be easier to silence the glycerol production gene.
APPEARS IN
RELATED QUESTIONS
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Expand the Given and mention one application of each:
PCR
Consult the internet and find out how to make orally active protein pharmaceuticals. What is the major problem to be encountered?
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
The first clinical gene therapy was done for the treatment of ________.
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
Which one of the following statements is true regarding DNA polymerase used in PCR?
ELISA is mainly used for ____________.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
Explain how recombinant Insulin can be produced.
Which of the following is a neuropeptide hormone?
Which is false about antibiotics?
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
The Adenosine deaminase deficiency results in ______.
How is a mature, functional insulin hormone different from its prohormone form?
List the advantages of recombinant insulin.
A cell-free method of amplifying DNA first developed in the mid 1980's revolutionised the field of biotechnology, Name the method and explain the basic steps of the technique involved.
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
Give a reason for the following:
Streptokinase is administered to the patients having myocardial infarction.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Illustrate using any one example of transgenic bacteria.
