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Question
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
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Solution
- Restriction endonucleases: for cutting the desired DNA at desired places.
- Gel electrophoresis: for separating the desired DNA fragments.
- Ligase enzyme: for creating recombinant DNA molecule.
- DNA delivery system: like electroporation, microinjection, gene gun method.
- Competent host (usually bacteria/yeast): to take up recombinant DNA.
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