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प्रश्न
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
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उत्तर
- Restriction endonucleases: for cutting the desired DNA at desired places.
- Gel electrophoresis: for separating the desired DNA fragments.
- Ligase enzyme: for creating recombinant DNA molecule.
- DNA delivery system: like electroporation, microinjection, gene gun method.
- Competent host (usually bacteria/yeast): to take up recombinant DNA.
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संबंधित प्रश्न
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
The first clinical gene therapy was done for the treatment of ________.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
How is the amplification of a gene sample of interest carried out using PCR?
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
The site of production of ADA the body is ______.
A pathophysiology is the ______.
ELISA is based on:
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
List the advantages of recombinant insulin.
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Illustrate using any one example of transgenic bacteria.
