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प्रश्न
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
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उत्तर
- Restriction endonucleases: for cutting the desired DNA at desired places.
- Gel electrophoresis: for separating the desired DNA fragments.
- Ligase enzyme: for creating recombinant DNA molecule.
- DNA delivery system: like electroporation, microinjection, gene gun method.
- Competent host (usually bacteria/yeast): to take up recombinant DNA.
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संबंधित प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
What is gene therapy? Name the first clinical case in which it was used.
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
Describe the role of high temperature.
The first clinical gene therapy was done for the treatment of ________.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
What are DNA vaccines?
What are recombinant vaccines? Explain the types.
A patient suffering from diabetes mellitus will have ______.
Which is false about antibiotics?
The Adenosine deaminase deficiency results in ______.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
Illustrate using any one example of transgenic bacteria.
