Advertisements
Advertisements
प्रश्न
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
Advertisements
उत्तर
The specificity and sensitivity of PCR is useful for the diagnosis of inherited disorders (genetic diseases), viral diseases, bacterial diseases, etc., The diagnosis and treatment of a particular disease often require identifying a particular pathogen. Traditional methods of identification involve culturing these organisms from clinical specimens and performing metabolic and other tests to identify them. The concept behind PCR-based diagnosis of infectious diseases is simple - if the pathogen is present in a clinical specimen its DNA will be present.
Its DNA has unique sequences that can be detected by PCR, often using the clinical specimen (for example, blood, stool, spinal fluid, or sputum) in the PCR mixture.
APPEARS IN
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
Compare and contrast the advantages and disadvantages of the production of genetically modified crops.
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
Expand the Given and mention one application of each:
ELISA
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
How many amino acids are arranged in the two chains of Insulin?
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
Explain how recombinant Insulin can be produced.
A patient suffering from diabetes mellitus will have ______.
The hormone insulin is secreted by:
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
ELISA is based on:
The Adenosine deaminase deficiency results in ______.
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
