Advertisements
Advertisements
प्रश्न
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
Advertisements
उत्तर
The specificity and sensitivity of PCR is useful for the diagnosis of inherited disorders (genetic diseases), viral diseases, bacterial diseases, etc., The diagnosis and treatment of a particular disease often require identifying a particular pathogen. Traditional methods of identification involve culturing these organisms from clinical specimens and performing metabolic and other tests to identify them. The concept behind PCR-based diagnosis of infectious diseases is simple - if the pathogen is present in a clinical specimen its DNA will be present.
Its DNA has unique sequences that can be detected by PCR, often using the clinical specimen (for example, blood, stool, spinal fluid, or sputum) in the PCR mixture.
APPEARS IN
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and the chemistry of oil?
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
Expand the Given and mention one application of each:
ELISA
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Give a schematic representation of the transformation of a pro-insulin into insulin.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
Explain how “Rosie” is different from a normal cow.
A patient suffering from diabetes mellitus will have ______.
C-peptide of human insulin is ______.
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
ELISA is based on:
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
