Advertisements
Advertisements
प्रश्न
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
Advertisements
उत्तर
The specificity and sensitivity of PCR is useful for the diagnosis of inherited disorders (genetic diseases), viral diseases, bacterial diseases, etc., The diagnosis and treatment of a particular disease often require identifying a particular pathogen. Traditional methods of identification involve culturing these organisms from clinical specimens and performing metabolic and other tests to identify them. The concept behind PCR-based diagnosis of infectious diseases is simple - if the pathogen is present in a clinical specimen its DNA will be present.
Its DNA has unique sequences that can be detected by PCR, often using the clinical specimen (for example, blood, stool, spinal fluid, or sputum) in the PCR mixture.
APPEARS IN
संबंधित प्रश्न
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
State the role of C-peptide in human insulin.
Expand the Given and mention one application of each:
ELISA
Explain how insulin can be produced using recombinant DNA technology.
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
How many amino acids are arranged in the two chains of Insulin?
If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
Human insulin is being commercially produced forma transgenic species of ______.
Which is false about antibiotics?
ELISA is based on:
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
Can a disease be detected before its symptoms appear? Explain the principle involved.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Discuss briefly how a probe is used in molecular diagnostics.
How is a mature, functional insulin hormone different from its prohormone form?
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
Atherosclerosis can be treated with recombinant protein called ______
