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प्रश्न
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
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उत्तर
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
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संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
Which of the following is a neuropeptide hormone?
C-peptide of human insulin is ______.
A pathophysiology is the ______.
Taking examples under each category, discuss upstream and downstream processing.
A host cell must be made competent, before it is able to receive an rDNA. Justify.
