Advertisements
Advertisements
प्रश्न
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Advertisements
उत्तर
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
APPEARS IN
संबंधित प्रश्न
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
How many amino acids are arranged in the two chains of Insulin?
Which one of the following statements is true regarding DNA polymerase used in PCR?
What are recombinant vaccines? Explain the types.
The hormone insulin is secreted by:
Discuss briefly how a probe is used in molecular diagnostics.
Taking examples under each category, discuss upstream and downstream processing.
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
