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प्रश्न
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
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उत्तर
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
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संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
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Describe the role of primers.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
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The ______ gene from Bacillus thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.
