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प्रश्न
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
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उत्तर
Genetically Engineered Insulin:
- Insulin contains two short polypeptide chains: chain A and chain B linked together by disulphide bridges.
- In mammals, insulin is synthesized as a pro-hormone. It contains an extra stretch called C-peptide.
- C-peptide is absent in the mature insulin and is removed during maturation into insulin.
- Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly in 1983. It prepared two DNA sequences corresponding to A and B, chains of human insulin, and introduced them in plasmids of E. coli for production.
The A and B chains produced were separated, extracted, and combined, by creating disulfide bonds to form human insulin.
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संबंधित प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Expand the Given and mention one application of each:
ELISA
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
How is the amplification of a gene sample of interest carried out using PCR?
A patient suffering from diabetes mellitus will have ______.
The site of production of ADA the body is ______.
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
How is a mature, functional insulin hormone different from its prohormone form?
A patient is suffering from ADA deficiency. Can he be cured? How?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
