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प्रश्न
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
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उत्तर
Genetically Engineered Insulin:
- Insulin contains two short polypeptide chains: chain A and chain B linked together by disulphide bridges.
- In mammals, insulin is synthesized as a pro-hormone. It contains an extra stretch called C-peptide.
- C-peptide is absent in the mature insulin and is removed during maturation into insulin.
- Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly in 1983. It prepared two DNA sequences corresponding to A and B, chains of human insulin, and introduced them in plasmids of E. coli for production.
The A and B chains produced were separated, extracted, and combined, by creating disulfide bonds to form human insulin.
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संबंधित प्रश्न
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
State the best-known contribution of P.K. Sethi
Describe the role of high temperature.
Describe the role of primers.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
Explain how recombinant Insulin can be produced.
Choose the correct option regarding retrovirus.
ELISA is based on:
The Adenosine deaminase deficiency results in ______.
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
