Advertisements
Advertisements
प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Advertisements
उत्तर
The structure of insulin consists of two short polypeptide chains chain A and chain B, linked together by disulphide bridges. In mammals, including human beings, insulin is synthesised as a pro-hormone, which contains an extra stretch called the C peptide, which is removed during maturation into insulin. The rDNA technique is used for assembling insulin into the mature form.
In 1983, Eli Lilly, an American company, prepared two DNA sequences corresponding to the A and B chains of human insulin and introduced them into the plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
APPEARS IN
संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Give a schematic representation of the transformation of a pro-insulin into insulin.
Which one of the following statements is true regarding DNA polymerase used in PCR?
ELISA is mainly used for ____________.
Explain how “Rosie” is different from a normal cow.
Explain how ADA deficiency can be corrected?
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
Which of the following is a neuropeptide hormone?
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
What is a recombinant DNA vaccine? Give two examples.
How is a mature, functional insulin hormone different from its prohormone form?
A patient is suffering from ADA deficiency. Can he be cured? How?
In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.
Give a reason for the following:
Streptokinase is administered to the patients having myocardial infarction.
Illustrate using any one example of transgenic bacteria.
