Advertisements
Advertisements
प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Advertisements
उत्तर
The structure of insulin consists of two short polypeptide chains chain A and chain B, linked together by disulphide bridges. In mammals, including human beings, insulin is synthesised as a pro-hormone, which contains an extra stretch called the C peptide, which is removed during maturation into insulin. The rDNA technique is used for assembling insulin into the mature form.
In 1983, Eli Lilly, an American company, prepared two DNA sequences corresponding to the A and B chains of human insulin and introduced them into the plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
APPEARS IN
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Why do children cured by enzyme-replacement therapy adenosine deaminase deficiency need periodic treatment?
Write the function of adenosine deaminase enzyme.
What is gene therapy? Name the first clinical case in which it was used.
Give a schematic representation of the transformation of a pro-insulin into insulin.
Describe the role of primers.
What are recombinant vaccines? Explain the types.
A patient suffering from diabetes mellitus will have ______.
Which of the following is a neuropeptide hormone?
Human insulin is being commercially produced forma transgenic species of ______.
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
The Adenosine deaminase deficiency results in ______.
Can a disease be detected before its symptoms appear? Explain the principle involved.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
How is a mature, functional insulin hormone different from its prohormone form?
A patient is suffering from ADA deficiency. Can he be cured? How?
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.
In the recombinant production of humulin using Escherichia coli, which plasmid vector and bacterial gene are fused with the human insulin A and B chain sequences?
Which of the following describes the final step in the laboratory synthesis of biologically active humulin from isolated chains?
