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प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
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उत्तर
The structure of insulin consists of two short polypeptide chains chain A and chain B, linked together by disulphide bridges. In mammals, including human beings, insulin is synthesised as a pro-hormone, which contains an extra stretch called the C peptide, which is removed during maturation into insulin. The rDNA technique is used for assembling insulin into the mature form.
In 1983, Eli Lilly, an American company, prepared two DNA sequences corresponding to the A and B chains of human insulin and introduced them into the plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
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संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Expand the Given and mention one application of each:
PCR
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
What is gene therapy? Name the first clinical case in which it was used.
Describe the role of high temperature.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
The site of production of ADA the body is ______.
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Discuss briefly how a probe is used in molecular diagnostics.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
A patient is suffering from ADA deficiency. Can he be cured? How?
List the advantages of recombinant insulin.
A host cell must be made competent, before it is able to receive an rDNA. Justify.
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Choose the INCORRECT statement.
Why is the Polymerase Chain Reaction (PCR) preferred over conventional culture and serological methods for early disease diagnosis?
