Advertisements
Advertisements
Question
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Advertisements
Solution
The structure of insulin consists of two short polypeptide chains chain A and chain B, linked together by disulphide bridges. In mammals, including human beings, insulin is synthesised as a pro-hormone, which contains an extra stretch called the C peptide, which is removed during maturation into insulin. The rDNA technique is used for assembling insulin into the mature form.
In 1983, Eli Lilly, an American company, prepared two DNA sequences corresponding to the A and B chains of human insulin and introduced them into the plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin.
APPEARS IN
RELATED QUESTIONS
State the role of C-peptide in human insulin.
What is gene therapy? Name the first clinical case in which it was used.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Describe the role of primers.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
The first clinical gene therapy was done for the treatment of ________.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
Choose the correct option regarding retrovirus.
ELISA is based on:
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as ______
Now a days it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize its complimentary DNA in a clone of cells, followed by its detection using autoradiography because ______
The Adenosine deaminase deficiency results in ______.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Taking examples under each category, discuss upstream and downstream processing.
How is a mature, functional insulin hormone different from its prohormone form?
A patient is suffering from ADA deficiency. Can he be cured? How?
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
Give a reason for the following:
Streptokinase is administered to the patients having myocardial infarction.
