Advertisements
Advertisements
प्रश्न
Can a disease be detected before its symptoms appear? Explain the principle involved.
Advertisements
उत्तर
Yes, detection of disease before its symptoms appear can be possible by only amplifying the nucleic acids by the technique known as Polymerase Chain Reaction (PCR), which was not possible with conventional diagnostic tests. A single DNA molecule can be copied endlessly using Primers, DNA polymerase enzyme, and free nucleotides under appropriate conditions in PCR.
APPEARS IN
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Why do children cured by enzyme-replacement therapy adenosine deaminase deficiency need periodic treatment?
Write the function of adenosine deaminase enzyme.
What is gene therapy? Name the first clinical case in which it was used.
Give a schematic representation of the transformation of a pro-insulin into insulin.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
A pathophysiology is the ______.
ELISA is based on:
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
How is a mature, functional insulin hormone different from its prohormone form?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
In the recombinant production of humulin using Escherichia coli, which plasmid vector and bacterial gene are fused with the human insulin A and B chain sequences?
The first edible vaccine was developed in tobacco plants to express which specific antigen?
Which diagnostic technique utilizes antigen-antibody interactions coupled with an enzyme colour reaction, making it well-suited for rapid mass screening?
