Advertisements
Advertisements
प्रश्न
Illustrate using any one example of transgenic bacteria.
Advertisements
उत्तर
Genetic technology has prepared human insulin. Insulin is made up of two small polypeptide chains, chain ‘A’ and chain ‘B’ which are linked to each other by disulphide bonds. In human insulin, a pro-hormone is synthesised which contains C peptide. Mature insulin does not contain this peptide ‘C’; it separates from insulin during the maturation process. In 1983, scientists created two DNA sequences for the ‘A’ and ‘B’ chains of human insulin and put them into E. coli to make these insulin chains. We removed these separately produced chains ‘A’ and ‘B’ and joined them to each other by disulphide bonds to prepare human insulin. Insulin is a useful medicine for controlling diabetes. The credit for cloning the insulin gene goes to Dr. Saran Narang of Indian origin. He conducted his experiment in Ottawa, Canada.
संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
State the best-known contribution of P.K. Sethi
Give a schematic representation of the transformation of a pro-insulin into insulin.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Describe the steps that are followed during secondary treatment of sewage.
Describe the role of high temperature.
How many amino acids are arranged in the two chains of Insulin?
What are DNA vaccines?
A pathophysiology is the ______.
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Can a disease be detected before its symptoms appear? Explain the principle involved.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
Define Antigen and Antibody. Name any two diagnostic kits based upon them.
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
Give a reason for the following:
Streptokinase is administered to the patients having myocardial infarction.
