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प्रश्न
Illustrate using any one example of transgenic bacteria.
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उत्तर
Genetic technology has prepared human insulin. Insulin is made up of two small polypeptide chains, chain ‘A’ and chain ‘B’ which are linked to each other by disulphide bonds. In human insulin, a pro-hormone is synthesised which contains C peptide. Mature insulin does not contain this peptide ‘C’; it separates from insulin during the maturation process. In 1983, scientists created two DNA sequences for the ‘A’ and ‘B’ chains of human insulin and put them into E. coli to make these insulin chains. We removed these separately produced chains ‘A’ and ‘B’ and joined them to each other by disulphide bonds to prepare human insulin. Insulin is a useful medicine for controlling diabetes. The credit for cloning the insulin gene goes to Dr. Saran Narang of Indian origin. He conducted his experiment in Ottawa, Canada.
संबंधित प्रश्न
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
State the role of C-peptide in human insulin.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
How many amino acids are arranged in the two chains of Insulin?
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
What is genetically engineered Insulin?
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
What are recombinant vaccines? Explain the types.
C-peptide of human insulin is ______.
The site of production of ADA the body is ______.
Choose the correct option regarding retrovirus.
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as ______
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Define Antigen and Antibody. Name any two diagnostic kits based upon them.
A host cell must be made competent, before it is able to receive an rDNA. Justify.
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Name the technique used to detect the presence of HIV in the body of an individual, Justify the principle associated with this technique.
