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प्रश्न
Illustrate using any one example of transgenic bacteria.
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उत्तर
Genetic technology has prepared human insulin. Insulin is made up of two small polypeptide chains, chain ‘A’ and chain ‘B’ which are linked to each other by disulphide bonds. In human insulin, a pro-hormone is synthesised which contains C peptide. Mature insulin does not contain this peptide ‘C’; it separates from insulin during the maturation process. In 1983, scientists created two DNA sequences for the ‘A’ and ‘B’ chains of human insulin and put them into E. coli to make these insulin chains. We removed these separately produced chains ‘A’ and ‘B’ and joined them to each other by disulphide bonds to prepare human insulin. Insulin is a useful medicine for controlling diabetes. The credit for cloning the insulin gene goes to Dr. Saran Narang of Indian origin. He conducted his experiment in Ottawa, Canada.
संबंधित प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Consult the internet and find out how to make orally active protein pharmaceuticals. What is the major problem to be encountered?
Expand the Given and mention one application of each:
ELISA
Give a schematic representation of the transformation of a pro-insulin into insulin.
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
Describe the role of primers.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
What is genetically engineered Insulin?
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
Explain how ADA deficiency can be corrected?
What are DNA vaccines?
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
What is a recombinant DNA vaccine? Give two examples.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Define Antigen and Antibody. Name any two diagnostic kits based upon them.
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
List the advantages of recombinant insulin.
A cell-free method of amplifying DNA first developed in the mid 1980's revolutionised the field of biotechnology, Name the method and explain the basic steps of the technique involved.
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
