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प्रश्न
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
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उत्तर
Enzyme Linked Immuno-sorbent Assay (ELISA) is used for the detection of AIDS. ELISA is based on the principle of antigen-antibody interaction. Infection by pathogen can be detected by the presence of antigens (proteins, glycoproteins, etc.) or by detecting the antibodies synthesised against the pathogen.
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संबंधित प्रश्न
Why do children cured by enzyme-replacement therapy adenosine deaminase deficiency need periodic treatment?
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Explain how insulin can be produced using recombinant DNA technology.
State the best-known contribution of P.K. Sethi
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Describe the role of primers.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
The hormone insulin is secreted by:
Human insulin is being commercially produced form a transgenic species of ______.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
How is a mature, functional insulin hormone different from its prohormone form?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The ______ gene from Bacillus thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.
Globally, approximately how many recombinant therapeutics have been approved for human use, and how many of them are currently marketed in India?
What is the structural difference between proinsulin and mature, biologically active insulin?
