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प्रश्न
Explain how insulin can be produced using recombinant DNA technology.
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उत्तर
Production of insulin using recombinant DNA technology:
- Extraction of mRNA (coding for insulin) from pancreas cell from human (donor)
- With the help of reverse transcriptase making copy of DNA (c DNA) (coding for insulin)
- Extraction of plasmid from bacterial cell.
- Opening plasmid using restriction endonuclease.
- Insertion of cDNA in plasmid and sealing it using DNA ligase.
- Reintroduction of plasmid vector (recombinant vector) into bacterial cell (a competent host cell)
- Culturing these cells to obtain multiple copies or clones of derised fragmant of DNA (cDNA).
- Replication of plasmid and production of human mRNA which instructs the cell to make insulin (Humulin)
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संबंधित प्रश्न
What is genetically engineered Insulin?
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
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Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Now a days it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize its complimentary DNA in a clone of cells, followed by its detection using autoradiography because ______
Can a disease be detected before its symptoms appear? Explain the principle involved.
What is a recombinant DNA vaccine? Give two examples.
Discuss briefly how a probe is used in molecular diagnostics.
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
