Advertisements
Advertisements
प्रश्न
Explain how insulin can be produced using recombinant DNA technology.
Advertisements
उत्तर
Production of insulin using recombinant DNA technology:
- Extraction of mRNA (coding for insulin) from pancreas cell from human (donor)
- With the help of reverse transcriptase making copy of DNA (c DNA) (coding for insulin)
- Extraction of plasmid from bacterial cell.
- Opening plasmid using restriction endonuclease.
- Insertion of cDNA in plasmid and sealing it using DNA ligase.
- Reintroduction of plasmid vector (recombinant vector) into bacterial cell (a competent host cell)
- Culturing these cells to obtain multiple copies or clones of derised fragmant of DNA (cDNA).
- Replication of plasmid and production of human mRNA which instructs the cell to make insulin (Humulin)
APPEARS IN
संबंधित प्रश्न
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Consult the internet and find out how to make orally active protein pharmaceuticals. What is the major problem to be encountered?
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
Describe the role of high temperature.
Which one of the following statements is true regarding DNA polymerase used in PCR?
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
Explain how “Rosie” is different from a normal cow.
ELISA is based on:
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
