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Question
Explain how insulin can be produced using recombinant DNA technology.
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Solution
Production of insulin using recombinant DNA technology:
- Extraction of mRNA (coding for insulin) from pancreas cell from human (donor)
- With the help of reverse transcriptase making copy of DNA (c DNA) (coding for insulin)
- Extraction of plasmid from bacterial cell.
- Opening plasmid using restriction endonuclease.
- Insertion of cDNA in plasmid and sealing it using DNA ligase.
- Reintroduction of plasmid vector (recombinant vector) into bacterial cell (a competent host cell)
- Culturing these cells to obtain multiple copies or clones of derised fragmant of DNA (cDNA).
- Replication of plasmid and production of human mRNA which instructs the cell to make insulin (Humulin)
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