Advertisements
Advertisements
Question
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Advertisements
Solution
Enzyme Linked Immuno-sorbent Assay (ELISA) is used for the detection of AIDS. ELISA is based on the principle of antigen-antibody interaction. Infection by pathogen can be detected by the presence of antigens (proteins, glycoproteins, etc.) or by detecting the antibodies synthesised against the pathogen.
APPEARS IN
RELATED QUESTIONS
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
What is genetically engineered Insulin?
Explain how “Rosie” is different from a normal cow.
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
Explain how ADA deficiency can be corrected?
Explain how recombinant Insulin can be produced.
Human insulin is being commercially produced forma transgenic species of ______.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
The site of production of ADA the body is ______.
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
Can a disease be detected before its symptoms appear? Explain the principle involved.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Choose the INCORRECT statement.
Globally, approximately how many recombinant therapeutics have been approved for human use, and how many of them are currently marketed in India?
Which type of vaccine uses an oral formulation produced using Bacillus subtilis?
Why is the Polymerase Chain Reaction (PCR) preferred over conventional culture and serological methods for early disease diagnosis?
