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प्रश्न
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
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उत्तर
Enzyme Linked Immuno-sorbent Assay (ELISA) is used for the detection of AIDS. ELISA is based on the principle of antigen-antibody interaction. Infection by pathogen can be detected by the presence of antigens (proteins, glycoproteins, etc.) or by detecting the antibodies synthesised against the pathogen.
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संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
Which one of the following statements is true regarding DNA polymerase used in PCR?
ELISA is mainly used for ____________.
What is genetically engineered Insulin?
Explain how “Rosie” is different from a normal cow.
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
Which is false about antibiotics?
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
The Adenosine deaminase deficiency results in ______.
A patient is suffering from ADA deficiency. Can he be cured? How?
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
The ______ gene from Bacillus thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.
In the recombinant production of humulin using Escherichia coli, which plasmid vector and bacterial gene are fused with the human insulin A and B chain sequences?
The first edible vaccine was developed in tobacco plants to express which specific antigen?
Which molecular diagnostic tool relies on the hybridisation of labelled single-stranded DNA to detect specific complementary sequences or mutant alleles?
