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प्रश्न
Taking examples under each category, discuss upstream and downstream processing.
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उत्तर
Upstream processing: Biotechnological processes can be separated into upstream processes and downstream processes. The upstream process is defined as the entire process from DNA isolation and culture expansion of the cells until the final product. Downstream processing: After completion of the biosynthetic stage, the product has to be subjected to a series of processes before it Is ready for marketing as a finished product.
The processes include separation and purification, which are collectively referred to as downstream processing. The product has to be formulated with suitable preservatives. Such formulation has to undergo clinical trials as in the case of drugs. Strict quality control testing for each product is also required. The downstream processing and quality control testing vary from product to product.
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संबंधित प्रश्न
Explain how insulin can be produced using recombinant DNA technology.
What is gene therapy? Name the first clinical case in which it was used.
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Describe the role of high temperature.
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What are recombinant vaccines? Explain the types.
C-peptide of human insulin is ______.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
Choose the correct option regarding retrovirus.
Which is false about antibiotics?
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
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In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:

- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
Choose the INCORRECT statement.
