Advertisements
Advertisements
प्रश्न
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
Advertisements
उत्तर
- Restriction endonucleases: for cutting the desired DNA at desired places.
- Gel electrophoresis: for separating the desired DNA fragments.
- Ligase enzyme: for creating recombinant DNA molecule.
- DNA delivery system: like electroporation, microinjection, gene gun method.
- Competent host (usually bacteria/yeast): to take up recombinant DNA.
APPEARS IN
संबंधित प्रश्न
Why do children cured by enzyme-replacement therapy adenosine deaminase deficiency need periodic treatment?
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
State the best-known contribution of P.K. Sethi
Describe the role of primers.
The first clinical gene therapy was done for the treatment of ________.
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
How is the amplification of a gene sample of interest carried out using PCR?
If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
What are recombinant vaccines? Explain the types.
A patient suffering from diabetes mellitus will have ______.
C-peptide of human insulin is ______.
A pathophysiology is the ______.
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
