Advertisements
Advertisements
प्रश्न
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
Advertisements
उत्तर
- Restriction endonucleases: for cutting the desired DNA at desired places.
- Gel electrophoresis: for separating the desired DNA fragments.
- Ligase enzyme: for creating recombinant DNA molecule.
- DNA delivery system: like electroporation, microinjection, gene gun method.
- Competent host (usually bacteria/yeast): to take up recombinant DNA.
APPEARS IN
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
Consult the internet and find out how to make orally active protein pharmaceuticals. What is the major problem to be encountered?
State the best-known contribution of P.K. Sethi
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Describe the role of high temperature.
Which one of the following statements is true regarding DNA polymerase used in PCR?
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
A pathophysiology is the ______.
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as ______
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Discuss briefly how a probe is used in molecular diagnostics.
A patient is suffering from ADA deficiency. Can he be cured? How?
A host cell must be made competent, before it is able to receive an rDNA. Justify.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Choose the INCORRECT statement.
