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प्रश्न
Describe the role of high temperature.
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उत्तर
The first step of PCR is denaturation. In this step, high temperature is required for denaturation of the DNA strands i.e. separating the double-stranded DNA into single strands. Since the two strands are held together by hydrogen bonding, a high temperature is required to break down these bonds and separate the two strands.
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