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प्रश्न
Illustrate using any one example of transgenic bacteria.
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उत्तर
Genetic technology has prepared human insulin. Insulin is made up of two small polypeptide chains, chain ‘A’ and chain ‘B’ which are linked to each other by disulphide bonds. In human insulin, a pro-hormone is synthesised which contains C peptide. Mature insulin does not contain this peptide ‘C’; it separates from insulin during the maturation process. In 1983, scientists created two DNA sequences for the ‘A’ and ‘B’ chains of human insulin and put them into E. coli to make these insulin chains. We removed these separately produced chains ‘A’ and ‘B’ and joined them to each other by disulphide bonds to prepare human insulin. Insulin is a useful medicine for controlling diabetes. The credit for cloning the insulin gene goes to Dr. Saran Narang of Indian origin. He conducted his experiment in Ottawa, Canada.
संबंधित प्रश्न
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
Explain how insulin can be produced using recombinant DNA technology.
Describe the role of primers.
How many amino acids are arranged in the two chains of Insulin?
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
Which one of the following statements is true regarding DNA polymerase used in PCR?
What is genetically engineered Insulin?
If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
A pathophysiology is the ______.
ELISA is based on:
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
What is a recombinant DNA vaccine? Give two examples.
Discuss briefly how a probe is used in molecular diagnostics.
How is a mature, functional insulin hormone different from its prohormone form?
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
A patient is suffering from ADA deficiency. Can he be cured? How?
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
