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प्रश्न
Illustrate using any one example of transgenic bacteria.
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उत्तर
Genetic technology has prepared human insulin. Insulin is made up of two small polypeptide chains, chain ‘A’ and chain ‘B’ which are linked to each other by disulphide bonds. In human insulin, a pro-hormone is synthesised which contains C peptide. Mature insulin does not contain this peptide ‘C’; it separates from insulin during the maturation process. In 1983, scientists created two DNA sequences for the ‘A’ and ‘B’ chains of human insulin and put them into E. coli to make these insulin chains. We removed these separately produced chains ‘A’ and ‘B’ and joined them to each other by disulphide bonds to prepare human insulin. Insulin is a useful medicine for controlling diabetes. The credit for cloning the insulin gene goes to Dr. Saran Narang of Indian origin. He conducted his experiment in Ottawa, Canada.
संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
Compare and contrast the advantages and disadvantages of the production of genetically modified crops.
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Expand the Given and mention one application of each:
ELISA
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
Vaccines that use components of a pathogenic organism rather than the whole organism are called ____________.
What is genetically engineered Insulin?
Explain how “Rosie” is different from a normal cow.
The hormone insulin is secreted by:
Which of the following is a neuropeptide hormone?
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
The site of production of ADA the body is ______.
Which is false about antibiotics?
ELISA is based on:
List the advantages of recombinant insulin.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
Which diagnostic technique utilizes antigen-antibody interactions coupled with an enzyme colour reaction, making it well-suited for rapid mass screening?
