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प्रश्न
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
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उत्तर
Yes. One can use antibody against the enzyme (that is responsible for the metabolism of phenylalanine) to develop ELISA based diagnostic technique. The patient where the enzyme protein is absent would give negative result in ELISA when compared to the normal individuals.
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संबंधित प्रश्न
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
Expand the Given and mention one application of each:
ELISA
Explain how insulin can be produced using recombinant DNA technology.
Give a schematic representation of the transformation of a pro-insulin into insulin.
Explain how ADA deficiency can be corrected?
Explain how recombinant Insulin can be produced.
Human insulin is being commercially produced forma transgenic species of ______.
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
Choose the correct option regarding retrovirus.
Which is false about antibiotics?
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
Discuss briefly how a probe is used in molecular diagnostics.
How is a mature, functional insulin hormone different from its prohormone form?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
Name the technique used to detect the presence of HIV in the body of an individual, Justify the principle associated with this technique.
