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Question
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
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Solution
1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.
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