Advertisements
Advertisements
Question
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
Advertisements
Solution
1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.
APPEARS IN
RELATED QUESTIONS
Why do children cured by enzyme-replacement therapy adenosine deaminase deficiency need periodic treatment?
Expand the Given and mention one application of each:
PCR
Consult the internet and find out how to make orally active protein pharmaceuticals. What is the major problem to be encountered?
ELISA is mainly used for ____________.
A pathophysiology is the ______.
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
What is a recombinant DNA vaccine? Give two examples.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
In the recombinant production of humulin using Escherichia coli, which plasmid vector and bacterial gene are fused with the human insulin A and B chain sequences?
What primary advantage do edible vaccines offer in terms of human immune response compared to traditional injected vaccines?
