Question

In the recombinant production of humulin using Escherichia coli, which plasmid vector and bacterial gene are fused with the human insulin A and B chain sequences?

Options

• Plasmid pBR322 fused with the tetracycline resistance promoter

• Ti plasmid fused with the kanamycin resistance gene

• Plasmid pUC19 fused with the ampicillin resistance gene

• Plasmid pBR322 fused with the \(\beta\)-galactosidase (\(\text{lacZ}\)) gene

Answer 1

The individual DNA sequences corresponding to the A and B chains of human insulin are inserted separately into the plasmid pBR322 alongside the \(\beta\)-galactosidase (\(\text{lacZ}\)) gene of \(E.\ coli\) to produce fusion proteins.