Advertisements
Advertisements
Question
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Advertisements
Solution
On finding the palindrome, the endonuclease binds to the DNA.
- It cuts the opposite strands of DNA, but between the same bases on both the strands & forms STICKY ENDS. This sticky ends facilitate the action of enzyme DNA ligase and help in the formation of recombination DNA.
APPEARS IN
RELATED QUESTIONS
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain briefly:
Restriction enzymes and DNA
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
What is elution?
State the importance of elution in this process.
