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प्रश्न
Which of the following statements does not hold true for restriction enzyme?
पर्याय
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It can produce the same kind of sticky ends in different DNA molecules
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उत्तर
It is isolated from viruses
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain briefly:
Restriction enzymes and DNA
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the principle involved in separation of DNA fragments using gel electrophoresis.
Identify the activity of endonuclease and exonuclease in the given image.
