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प्रश्न
Which of the following statements does not hold true for restriction enzyme?
पर्याय
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It can produce the same kind of sticky ends in different DNA molecules
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उत्तर
It is isolated from viruses
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Explain briefly:
Restriction enzymes and DNA
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Restriction enzymes ______.
'Restriction' in restriction enzyme refers to
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
How does one visualise DNA on an agarose gel?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What is elution?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
