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प्रश्न
Which of the following statements does not hold true for restriction enzyme?
पर्याय
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It can produce the same kind of sticky ends in different DNA molecules
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उत्तर
It is isolated from viruses
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Explain briefly:
Restriction enzymes and DNA
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Identify the activity of endonuclease and exonuclease in the given image.
What is the precise definition of a palindromic sequence in DNA?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
