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प्रश्न
Which of the following statements does not hold true for restriction enzyme?
पर्याय
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It can produce the same kind of sticky ends in different DNA molecules
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उत्तर
It is isolated from viruses
APPEARS IN
संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the importance of elution in this process.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
