English
Karnataka Board PUCPUC Science 2nd PUC Class 12

How does one visualise DNA on an agarose gel?

Advertisements
Advertisements

Question

How does one visualise DNA on an agarose gel?

Short/Brief Note
Advertisements

Solution

A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.

shaalaa.com
  Is there an error in this question or solution?
Chapter 11: Biotechnology : Principles and Processes - SHORT ANSWER [Page 79]

APPEARS IN

NCERT Exemplar Biology Exemplar [English] Class 12
Chapter 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 6. | Page 79

RELATED QUESTIONS

How are 'sticky ends' formed on a DNA strand? Why are they so called? 


Suggest a technique to a researcher who needs to separate fragments of DNA.


Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease


Distinguish between exonuclease and endonuclease.


Give a reason why :
Single cloning site is preferred in a vector.


The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.

Applying Chargaff’s rule, what conclusion can be drawn?


There is a restriction endonudease called as EcoRI. What does co part in it stands for?


DNA fragments separate according to size through?


Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?


'Restriction' in Restriction enzyme refers to ______.


Would you choose an exonuclease while producing a recombinant DNA molecule?


What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?


A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?


CTTAAG
GAATTC

  1. What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
  2. What is their significance in biotechnology?

Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.

(a) What result will be obtained on staining with ethidium bromide? Explain with reason.

(b) The above setup was modified and a band with 250 base pairs was obtained at X.

What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?


Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing 

  1. Anode end
  2. smallest/lightest DNA strand in the matrix
  3. Agarose gel


Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.

\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]

\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]

Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.


State the principle involved in separation of DNA fragments using gel electrophoresis.


Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×