Advertisements
Advertisements
Question
How does a restriction nuclease function? Explain
Advertisements
Solution
Restriction endonuclease functions by recognising its specific sequence. It binds to the DNA and cuts each to the two strands of the double helix at specific points in their sugar phosphate backbones. These enzymes cut the strand of DNA between the same two bases on the opposite strands leaving a single-stranded portion at their ends. These overhanging stretches are called sticky ends on each strand. The enzyme DNA ligase functions depending on the stickiness of these ends. For example, the restriction endonuclease EcoRI found in the colon bacteria E. coli recognises the base sequence GAATTC in the DNA duplex and cuts its strands between G and A.
APPEARS IN
RELATED QUESTIONS
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Do eukaryotic cells have restriction endonucleases? Justify your answer.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
State the importance of elution in this process.
