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Questions
Would you choose an exonuclease while producing a recombinant DNA molecule?
Would you like to choose an exonuclease enzyme while producing a recombinant DNA molecule?
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Solution
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
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