Advertisements
Advertisements
Question
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Advertisements
Solution
| Recombinant protein | Therapeutic use |
| Insulin | Treatment for type I diabetes mellitus. |
| Interferon-α | Used for chronic hepatitis C. |
| Interferon-α | Used against herpes and viral enteritis. |
| Coagulation factor VIII | Treatment of haemophilia A. |
| Coagulation factor IX | Treatment of haemophilia B. |
| DNAase I | Treatment of cystic fibrosis. |
| Anti-thrombin III | Prevention of blood clot. |
| Interferon B | For treatment of multiple sclerosis. |
| Human recombinant growth hormone | For promoting growth in an individual. |
| Tissue plasminogen activator | Treatment of acute myocardial infarction. |
APPEARS IN
RELATED QUESTIONS
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
Explain briefly:
Chitinase
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
______ plays an important role in blood clotting.
PCR and restriction fragment length polymorphism are the methods for ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
The first step in recombinant DNA technology is
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
How is copy number of the plasmid vector related to yield of recombinant protein?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
