Advertisements
Advertisements
Question
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Advertisements
Solution
| Recombinant protein | Therapeutic use |
| Insulin | Treatment for type I diabetes mellitus. |
| Interferon-α | Used for chronic hepatitis C. |
| Erythropoietin | Treatment of anemia. |
| Coagulation factor VIII | Treatment of haemophilia A. |
| Coagulation factor IX | Treatment of haemophilia B. |
| DNAase I | Treatment of cystic fibrosis. |
| Anti-thrombin III | Prevention of blood clots. |
| Interferon-β | For the treatment of multiple sclerosis. |
| Human recombinant growth hormone | For promoting growth in an individual. |
| Tissue plasminogen activator | Treatment of acute myocardial infarction. |
APPEARS IN
RELATED QUESTIONS
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Explain briefly:
PCR
Explain briefly:
Chitinase
State the importance of using a bioreactor.
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
Gene Amplification using primers can be done by ______.
The first step in recombinant DNA technology is
In PCR, primers are used for ______.
Rising of dough is due to ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
