English
Karnataka Board PUCPUC Science 2nd PUC Class 12

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).

Advertisements
Advertisements

Question

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).

Short Answer
Advertisements

Solution

Recombinant protein Therapeutic use
Insulin Treatment for type I diabetes mellitus.
Interferon-α Used for chronic hepatitis C.
Erythropoietin Treatment of anemia.
Coagulation factor VIII Treatment of haemophilia A.
Coagulation factor IX Treatment of haemophilia B.
DNAase I Treatment of cystic fibrosis.
Anti-thrombin III Prevention of blood clots.
Interferon-β For the treatment of multiple sclerosis.
Human recombinant growth hormone For promoting growth in an individual.
Tissue plasminogen activator Treatment of acute myocardial infarction.
shaalaa.com
  Is there an error in this question or solution?
Chapter 9: Biotechnology : Principles and Processes - EXERCISES [Page 175]

APPEARS IN

NCERT Biology [English] Class 12
Chapter 9 Biotechnology : Principles and Processes
EXERCISES | Q 1. | Page 175
Nootan Biology [English] Class 12 ISC
Chapter 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 1. | Page 535

RELATED QUESTIONS

Name two commonly used bioreactors.


Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Explain briefly:

Chitinase


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


PCR and restriction fragment length polymorphism are the methods for ______.


Gene Amplification using primers can be done by ______.


Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Rising of dough is due to ______.


An antibiotic resistance gene in a vector usually helps in the selection of ______.


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


How is copy number of the plasmid vector related to yield of recombinant protein?


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

State the advantage of using Thermostable DNA polymerase.


Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

  1. Insertion of recombinant DNA into the host cell.
  2. Cutting of DNA at specific location by restriction enzyme.
  3. Isolation of desired DNA fragment.
  4. Amplification of gene of interest using PCR. 

Choose the correct answer from the options given below:


Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×