Advertisements
Advertisements
Question
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

Advertisements
Solution
A - Denaturation
B - Annealing
C - Extension of primers
APPEARS IN
RELATED QUESTIONS
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Explain briefly:
Chitinase
Describe the process of amplification of the “gene of interest” using the PCR technique.
Which of the following term used for defining a viral genome incorporated into host DNA?
______ plays an important role in blood clotting.
PCR and restriction fragment length polymorphism are the methods for ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
Rising of dough is due to ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
What is the primary definition of recombinant DNA technology?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
