English
Karnataka Board PUCPUC Science 2nd PUC Class 12

Name the regions marked A, B and C. - Biology

Advertisements
Advertisements

Question

Name the regions marked A, B and C.

Diagram
Advertisements

Solution

A - Bam HI

B - Pst I

C - Ampicillin resistance gene (ampR)

shaalaa.com
Tools of Recombinant DNA Technology - Cloning Vectors
  Is there an error in this question or solution?
Chapter 11: Biotechnology : Principles and Processes - SHORT ANSWER [Page 80]

APPEARS IN

NCERT Exemplar Biology [English] Class 12
Chapter 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 12. | Page 80

RELATED QUESTIONS

Name the selectable markers in the cloning vector pBR322. Mention the role they play.


State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.


Describe briefly the following:

Origin of replication


Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?


Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.


Answer the following question.
Expand ‘YAC’ and mention what it was used for.


How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.


The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.


Which of the following statement is not true for a clone?


A suitable vector for gene cloning in higher organism is ______.


Plasmids are suitable vectors for gene cloning because these ______.


What does ‘competent’ refer to in competent cells used in transformation experiments?


A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?


Describe the role of CaCl2 in the preparation of competent cells?


For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.


Describe the role of Agrobacterium tumefaciens in transforming a plant cell.


The figure below shows the structure of a plasmid.

A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.

Choose the correct observation for the growth of bacterial colonies from the given table.


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×