Advertisements
Advertisements
Question
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Options
Competent bacterial cells
Transformed bacterial cells
Recombinant bacterial cells
None of the above
Advertisements
Solution
An antibiotic resistance gene in a vector usually helps in the selection of transformed bacterial cells.
APPEARS IN
RELATED QUESTIONS
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Which of the following term used for defining a viral genome incorporated into host DNA?
______ plays an important role in blood clotting.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
The first step in recombinant DNA technology is
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
