Advertisements
Advertisements
प्रश्न
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
Advertisements
उत्तर
The flow chart shows the three steps involved in the process of PCR showing the following -
- Denaturation The DNA strands are treated with a temperature of 94°C (Heat) and the strands are separated.
- Annealing The primers anneal to the complementary strands
- Extension The DNA polymerase facilitates the extension of the strands.
OR
Polymerase Chain Reaction
APPEARS IN
संबंधित प्रश्न
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Explain briefly:
PCR
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
