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प्रश्न
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
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उत्तर
No, biomolecules like DNA and protein cannot exhibit biological activity in anhydrous conditions. Hence, life is unsustainable without water.
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संबंधित प्रश्न
Name two commonly used bioreactors.
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
Describe the process of amplification of the “gene of interest” using the PCR technique.
______ plays an important role in blood clotting.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
What is the primary definition of recombinant DNA technology?
Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
