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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
Write a short note on Electrophoresis.
What are the restriction endonucleases? Give the rules of their nomenclature.
Describe two vectors less methods of gene transfer used in rDNA technology.
The bacterium which causes a plant disease called crown gall is _______________.
In vitro amplification of DNA or RNA segment is known as ___________.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Define biotechnology? Which are the basic principles and process of biotechnology?
Which of the following is TRUE regarding EcoR I?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Consider there is only one double stranded DNA molecule initially along with all other requirements of PCR. If a PCR machines performs 10 cycles of reaction then bow many double stranded DNA molecules would be present at the end?
____________ cuts the DNA within the specific positions.
______ is a Type I restriction enzyme.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Which of the following statements is correct with respect to cellular respiration?
Which of the following viruses has the capacity to produce lysozymes?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
What is polymerase chain reaction (PCR)?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
How does EcoRI differ from an exonuclease?
Modern biotechnology is based on two core techniques. Which of the following correctly identifies both of these techniques?
What is the function of DNA ligase in the creation of recombinant DNA?
Which of the following best describes the relationship between genetic engineering and recombinant DNA?
How does the concept of cloning relate to the multiplication of genetic material in biotechnology?
