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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Distinguish between RNA and DNA.
What are the restriction endonucleases? Give the rules of their nomenclature.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
What is the role of Taq-polymerase in PCR technology?
Explain types of enzymes used in biotechnology?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
From the following identify the methods of electrophoresis.
Which of the following is an INCORRECT match?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
A hind of biotechnology involving manipulation of DNA is ______.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
What is meant by gene cloning?
Which vector is mostly used in rDNA technology in plants?
Which of these are most widely used in genetic engineering?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
To be a cloning vector, a plasmid require ______.
Plasmid is ______.
What are the different types of electrophoresis?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Name the enzymes that are called molecular scissors.
How many sets of primers are required in each cycle of PCR?
Give a reason for the following:
DNA cannot enter directly into the host cell.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
