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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Write a short note on Electrophoresis.
Give one significant difference between:
Electroporation and Gene Gun.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Ti plasmid being used for introducing genes in plants obtained from ______.
What is Palindromic sequence?
Define biotechnology? Which are the basic principles and process of biotechnology?
What is Recognition sequence? Explain in brief.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Which of the following is TRUE regarding EcoR I?
Identify the CORRECT sequence of steps in PCR.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Which of the following device is required for PCR?
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
EcoRl & Hind III are the examples of ______
Human insulin is produced by culturing the genetically engineered ______
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
A hind of biotechnology involving manipulation of DNA is ______.
What is true for plasmid?
What is meant by gene cloning?
Annealing step of PCR operates at ______ °C.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
The PCR technique is used for ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write steps involved in gene amplification.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
