Advertisements
Advertisements
प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
Advertisements
उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
APPEARS IN
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Give an example of the source of thermostable enzyme DNA polymerase.
In vitro amplification of DNA or RNA segment is known as ___________.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Polymerase chain reaction is most useful in ______.
Alu-I is obtained from which organism?
What is the role of Taq-polymerase in PCR technology?
Bt-cotton shows adverse effect on the population of which butterfly?
Define biotechnology? Which are the basic principles and process of biotechnology?
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Lytic cycle involves bacteriophage and ____________.
Human insulin is produced by culturing the genetically engineered ______
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
DNA fingerprinting refers to ______.
What is meant by gene cloning?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Name two restriction enzymes used in PCR.
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
The DNA polymerase enzyme used in PCR is obtained from______.
