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प्रश्न
Name a recombinant vaccine that is currently being used in vaccination programme.
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उत्तर
Hepatitis B recombinant vaccine-engerix is used for vaccination of hepatitis virus.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
Expand the following abbreviation: BAC
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Why are bio-fertilizers preferred over chemical fertilizers?
What is the cell that receives a recombinant gene called?
Explain the properties of a good or ideal cloning vector for rDNA technology.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
What is plasmid?
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is an INCORRECT match?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Lytic cycle involves bacteriophage and ____________.
Name and describe the technique that helps in separation of DNA fragments.
Which one is a true statement regarding DNA polymerase used in PCR?
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Name two restriction enzymes used in PCR.
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
Describe three steps involved in mechanism of PCR.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Write a short note on vectors.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
Which one of the following enzymes is used to join DNA fragments?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
