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प्रश्न
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
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उत्तर
Flowchart showing formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI
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संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Explain briefly:
Chitinase
Describe the process of amplification of the “gene of interest” using the PCR technique.
______ plays an important role in blood clotting.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
