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प्रश्न
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
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उत्तर
Flowchart showing formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI
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संबंधित प्रश्न
Describe briefly the following:
Bioreactors
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Gene Amplification using primers can be done by ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
