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प्रश्न
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
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उत्तर
Flowchart showing formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI
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संबंधित प्रश्न
Can you recall meiosis and indicate at what stage recombinant DNA is made?
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?
How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?
