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प्रश्न
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
विकल्प
Easy availability of DNA template
Availability of synthetic primers
Availability of cheap deoxyribonucleotides
Availability of 'Thermostable' DNA polymerase
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उत्तर
Availability of 'Thermostable' DNA polymerase
APPEARS IN
संबंधित प्रश्न
Name two commonly used bioreactors.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
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A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
Rising of dough is due to ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
