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प्रश्न
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
विकल्प
Easy availability of DNA template
Availability of synthetic primers
Availability of cheap deoxyribonucleotides
Availability of 'Thermostable' DNA polymerase
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उत्तर
Availability of 'Thermostable' DNA polymerase
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संबंधित प्रश्न
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
State the importance of using a bioreactor.
Describe the process of amplification of the “gene of interest” using the PCR technique.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
PCR and restriction fragment length polymorphism are the methods for ______.
Gene Amplification using primers can be done by ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
State the advantage of using Thermostable DNA polymerase.
Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Which of the following statements is incorrect?
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
