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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

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प्रश्न

Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

विकल्प

  • Easy availability of DNA template

  • Availability of synthetic primers

  • Availability of cheap deoxyribonucleotides

  • Availability of 'Thermostable' DNA polymerase

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उत्तर

Availability of 'Thermostable' DNA polymerase

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अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७६]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 10. | पृष्ठ ७६

संबंधित प्रश्न

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe briefly the following:

Bioreactors


Describe the process of amplification of the “gene of interest” using the PCR technique.


______ plays an important role in blood clotting.


PCR and restriction fragment length polymorphism are the methods for ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


The first step in recombinant DNA technology is


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


How is copy number of the plasmid vector related to yield of recombinant protein?


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

  1. Insertion of recombinant DNA into the host cell.
  2. Cutting of DNA at specific location by restriction enzyme.
  3. Isolation of desired DNA fragment.
  4. Amplification of gene of interest using PCR. 

Choose the correct answer from the options given below:


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