Advertisements
Advertisements
प्रश्न
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
विकल्प
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
Extension of primer end on the template DNA
Advertisements
उत्तर
Extension of primer end on the template DNA
APPEARS IN
संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Describe briefly the following:
Bioreactors
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
The first step in recombinant DNA technology is
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Rising of dough is due to ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.

Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
Which series of operations is collectively referred to as 'downstream processing'?
