हिंदी
कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

Advertisements
Advertisements

प्रश्न

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

विकल्प

  • Denaturation of template DNA

  • Annealing of primers to template DNA

  • Extension of primer end on the template DNA

  • Extension of primer end on the template DNA

MCQ
Advertisements

उत्तर

Extension of primer end on the template DNA

shaalaa.com
  क्या इस प्रश्न या उत्तर में कोई त्रुटि है?
अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

APPEARS IN

एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 15. | पृष्ठ ७७

संबंधित प्रश्न

Name two commonly used bioreactors.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe briefly the following:

Downstream processing


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


Which of the following term used for defining a viral genome incorporated into host DNA?


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


In PCR, primers are used for ______.


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

  1. Insertion of recombinant DNA into the host cell.
  2. Cutting of DNA at specific location by restriction enzyme.
  3. Isolation of desired DNA fragment.
  4. Amplification of gene of interest using PCR. 

Choose the correct answer from the options given below:


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×