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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction? - Biology

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प्रश्न

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

विकल्प

  • Denaturation of template DNA

  • Annealing of primers to template DNA

  • Extension of primer end on the template DNA

  • Extension of primer end on the template DNA

MCQ
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उत्तर

Extension of primer end on the template DNA

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अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 15. | पृष्ठ ७७

संबंधित प्रश्न

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Explain briefly:

PCR


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


Which of the following term used for defining a viral genome incorporated into host DNA?


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

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