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प्रश्न
Rising of dough is due to ______.
विकल्प
Multiplication of yeast
Production of CO2
Emulsification
Hydrolysis of wheat flour starch into sugars
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उत्तर
Rising of dough is due to production of CO2.
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संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Describe briefly the following:
Bioreactors
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
Which of the following term used for defining a viral genome incorporated into host DNA?
PCR and restriction fragment length polymorphism are the methods for ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
The first step in recombinant DNA technology is
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
In PCR, primers are used for ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
How is copy number of the plasmid vector related to yield of recombinant protein?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
