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प्रश्न
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
विकल्प
Human gene may have intron which bacteria cannot process
Amino acid codons for humans and bacteria are different
Human protein is formed but degraded by bacteria
All of the above
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उत्तर
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be human gene may have intron which bacteria cannot process.
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संबंधित प्रश्न
Name two commonly used bioreactors.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Explain briefly:
PCR
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
The first step in recombinant DNA technology is
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Which of the following statements is incorrect?
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
What is the primary definition of recombinant DNA technology?
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
Which series of operations is collectively referred to as 'downstream processing'?
