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प्रश्न
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
पर्याय
Human gene may have intron which bacteria cannot process
Amino acid codons for humans and bacteria are different
Human protein is formed but degraded by bacteria
All of the above
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उत्तर
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be human gene may have intron which bacteria cannot process.
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संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Describe briefly the following:
Downstream processing
Explain briefly:
Chitinase
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
______ plays an important role in blood clotting.
PCR and restriction fragment length polymorphism are the methods for ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
Which of the following statements is incorrect?
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
Which series of operations is collectively referred to as 'downstream processing'?
