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Describe briefly the following: Downstream processing - Biology

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प्रश्न

Describe briefly the following:

Downstream processing

लघु उत्तरीय
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उत्तर

The product prepared by biotechnology is subjected to several processes before it is sent to the market. These processes include separation and purification and are collectively called downstream processing. The product is formulated with appropriate preservatives. In the case of drugs, such formulations are put through clinical trials. Every product also requires specific quality control tests. Downstream processing and quality control tests vary for different products.

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अध्याय 13: Principles and Processes of Biotechnology - NCERT EXERCISES WITH ANSWERS [पृष्ठ ५३५]

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नूतन Biology [English] Class 12 ISC
अध्याय 13 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 9. (iii) | पृष्ठ ५३५

संबंधित प्रश्न

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Explain briefly:

Chitinase


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


Which of the following term used for defining a viral genome incorporated into host DNA?


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


The first step in recombinant DNA technology is


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


In PCR, primers are used for ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Rising of dough is due to ______.


Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


State the advantage of using Thermostable DNA polymerase.


Which of the following statements is incorrect?


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