Advertisements
Advertisements
प्रश्न
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Advertisements
उत्तर
Small volume cultures cannot yield appreciable quantities of products. To produce in large quantities, the development of bioreactors, where large volumes (100-1000 litres) of culture can be processed, was required. Thus, bioreactors can be thought of as vessels in which raw materials are biologically converted into specific products, individual enzymes, etc., using microbial plant, animal or human cells. A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions (temperature, pH, substrate”, salts, vitamins, oxygen).
Simple stirred-tank bioreactor
Sparged stirred-tank bioreactor through which sterile air bubbles are sparged
| Flask | Bioreactor |
| Flask is used for small laboratory scale testing of a culture. | Bioreactor is used for commercial production |
| The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. | The cells can also be multiplied in a continuous culture system wherein the used medium is drained out from one side while the fresh medium is added from the other to maintain the cells in their physiologically most active log/exponential phase. This type of culturing method produces larger biomass leading to higher yields of desired protein. |
APPEARS IN
संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Explain briefly:
PCR
Explain briefly:
Chitinase
State the importance of using a bioreactor.
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Which of the following statements is incorrect?
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
